Highly multiplex guide RNA expression units of CRISPR/Cas9 were completely stable using cosmid amplification in a novel polygonal structure
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چکیده
منابع مشابه
a corpus-hased study of units of translation in english-persian literary translation
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15 صفحه اولMultiplex ligation-dependent probe amplification using a completely synthetic probe set.
The recent development of multiplex ligation-dependent probe amplification (MLPA) has provided an efficient and reliable assay for dosage screening of multiple loci in a single reaction. However, a drawback to this method is the time-consuming process of generating a probe set by cloning in single-stranded bacteriophage vectors. We have developed a synthetic probe set to screen for deletions in...
متن کاملA highly sensitive, multiplex immunoassay using gold nanoparticle-enhanced signal amplification.
We describe a highly sensitive, multiplex immunoassay utilizing gold nanoparticles for conjugation of detection antibodies with signal generators. The method has been compared with conventional methods and evaluated for simultaneous detection of two different biomarkers in human serum.
متن کاملA guide to ions and RNA structure.
RNA folding into stable tertiary structures is remarkably sensitive to the concentrations and types of cations present; an understanding of the physical basis of ion-RNA interactions is therefore a prerequisite for a quantitative accounting of RNA stability. This article summarizes the energetic factors that must be considered when ions interact with two different RNA environments. "Diffuse ion...
متن کاملthe role of thematic structure in comprehending spoken language
in fact this study is concerned with the relationship between the variation in thematice structure and the comprehension of spoken language. so the study focused on the following questions: 1. is there any relationship between thematic structure and the comprehension of spoken language? 2. which of the themes would have greated thematic force and be easier for the subjects to comprehend? accord...
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ژورنال
عنوان ژورنال: The Journal of Gene Medicine
سال: 2019
ISSN: 1099-498X,1521-2254
DOI: 10.1002/jgm.3115